Hi Everybody, I am testing neutrophil apoptosis in human whole blood after contact with various polymers used at biomaterials. I used AnnexinV assay with PI counterstaining and also TUNEL assay (without counterstaining). After 24 hours (which I found out to be a good timepoint,I get some results that totally confuse me. There is a group of polymers that cause high apoptosis rates in TUNEL assay but low rates in AnnexinV assay and also very low necrosis rates. These are the polymers that I know to be quite inert. On the other hand there are some polymers that strongly activate neutrophils (for example their receptor expression and IL-8 production). They show low apoptosis rates in TUNEL assay, high apoptosis rates in Annexin assay and low necrosis rates. These results are reproducible with a very low standard deviaton... But I simply cannot explain them! Does maybe anybody have a comment on this??? Thanks for your time! Silke Silke Schmidt Inst. f. Medizinische Mikrobiologie Klinikum der RWTH Aachen Pauwelsstraße 30 52074 Aachen Germany Tel: 0049-241-8088450 Fax: 0049-241-8082483 Email: sschmidt@ukaachen.de
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:25:55 EST