Hello! I am an absolute beginner in the FACS (and immunology) business and I am looking for advice on interpreting my FACS data. I have stained spleen and tumor cells using two antibody mixes: 1) CD4, CD8, CD3, NK 2) MHCI, MHCII, B7-1 The animals had been treated with an immunostimulating adenovirus intratumorally two weeks before sacrifice. I would like to know the relative numbers of CD4, CD8, NK, MHCI, MHCII and B7-1 positive cells compared to the control. As a control I only have samples from untreated animals (mock adenovirus). So far I know that cells double positive for CD4 and CD3 or for CD8 and CD3 can be counted as positive for CD4 or CD8 respectively. The double positive cells would be the upper right cell population. Unfortunately I don't know how to evaluate the rest of the data and I don't have anybody around who knows. Any advice on how to extract some information from such data as well as how to better design such staining experiments in the future is highly appreciated. Advice on where to get such information elsewhere or whether my question should be better posted in another forum is also welcome. By the way, the animals were not only sacrificed for the FACS analysis but managing this would make the other data much more valueable. Thanks for your help! With kind regards, Reinhard (waehler@uke.uni-hamburg.de)
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