Does anybody have experience in using Ig-Fc fragments to block Fc receptors in human B cells? We have tried to stain for surface FcR using FITC-human Ig Fc fragments, and get a minimal level of staining, so I don't even know if my unlabelled Fc fragments are binding. Martin S. Zand, MD, PhD Assistant Professor of Medicine and Medical Humanities Vox: 585.275.4517 Fax: 585.442.9201
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