We do sell R-PE conjugated anti-FITC http://www.probes.com/servlets/product?region=Select&item=21250 It should both shift the emission and enhance the fluorescence. the amount of enhancement will depend on the number of FITC targets but it should be significant, as shown below http://www.probes.com/handbook/figures/1631.html Sathiyaseelan wrote: > Hi > I have a FITC-conjugated peptide that binds to the surface of a cell line > and gives only a moderate intensity of FL1 signal (in 2nd decade) when > analyzed by flow cytometry. Unfortunately, I do not have a biotin-labelled > peptide in order to use a PE-strepavidin second reagent to amplify the > signal. Could any one tell me if there is a possibility to use an > anti-FITC antibody conjugated to PE as a second reagent to amplify the > fluorescent signal. How much signal amplification I could possibly achieve > using this PE conjugated-anti-FITC antibody method and can I use another > FITC-labeled antibody to label another surface antigen > simultaneously? Does anyone know a source of this antibody? I would > appreciate all useful info and help on this. > > Thanks > SATHI
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:09 EST