To all the Vantage users out there (and those doing lots of colours), We are (still) trying to set up our Vantage (DiVA) for >6 colours and we are wondering what people are using for "tweaking" alignments, voltages etc for far red and UV fluorochromes. Last time we checked with BD in Australia they didn't have any beads to recommend or sell us. We have tried with various stained cells but we are looking for something more reproducible for initial setup and optimisation. We are also interested in getting some idea of the settings people use and the levels we should be expecting from various flurochromes so we have a rough baseline from which to work. At this stage we are completely open to using any combination of fluorochromes - we just want to use more than 4! The Vantage is set up with three lasers (UV, 488, 633) and 8 detectors (2,4,2) or which 3 or 4 are supposed to be the far-red type. Any comments and suggestions would be most welcome! Thanks, Adrian -- ______________________________________________ Adrian Smith (Research Officer) T CELL BIOLOGY GROUP Centenary Institute of Cancer Medicine & Cell Biology Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. Ph: (02) 9565-6198 Fax: (02)-9565-6103
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