Howdy. I am observing a behavior from my MoFlo that isn't making sense to me, so I thought I'd pick some brains. This is what I've got when I use a 100 micron tip, 30-ish PSI, ~43kHz, ~4k trig'd events/sec, sorting out DCs: Concentrated sample, low sample differential, X events per second: High abort rate 30-50% Diluted sample, high sample differential to achieve the same (X) event rate: Low abort rate ~10% Purify 1-drop sort mode, and I'm using FSC pulse-width to 86 the aggregates. Aggregate percentages are the same whether I crank the diff. or let it trickle, so it doesn't seem to me to be a coincidence thing. Any thoughts, suggestions or strange looks are totally appreciated. Regards from San Diego, Nate Nate Regimbal IDEC Pharmaceuticals Corporation Cell Biology Group Ex. 8293
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