dear flowers,
I would like to hear comments on flow methods (using CFSE or any other dye)
that replace the traditinal thymidine assay. I am going to start doing LPA in HIV
infection and I have to decide between using the traditional assay and investing
some money to purchase a cell harvester, or trying a flow method. Flow cytometry is not
new to me but I dont have any experience with these new methods. Is there a method to
translate the flow data into an stimulation index similar to the one obtained inthe
thymidine assay?. Is it worth to try these new methods? I want to start analysing
samples tne next month?
Many thanks
Jose Benito
Hospital Carlos III
Madrid
Spain