Hello flowers! I have an investigator who has a population of mouse cells containing about 30% aneuploid cells, some with one chromosome missing and some with an extra one. He wants to sort (or otherwise process) the cells to get a relatively pure population of these two types. He wants to keep them alive and culture them afterwards. How feasible is this, in your opinion? Apart from using H33342, are there any other/better stains? - my investigator is concerned about the potential toxicity of Hoechst; I understood that it wasn't particularly toxic, but maybe I'm wrong? Yes, I have suggested limiting dilution cloning... this was met with a resounding silence! (also, a "clonal" population might not be particularly good, in this case). All advice gratefully received, grovel, grovel :-) - David [davidc@ccmi.salk.edu] [Salk Institute Flow Cytometry lab, La Jolla, CA, USA]
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:19 EST