Hi I have been using 5 mL K3 EDTA tubes for monkey flood collection for immunophenotyping. We have tested many animals and never had problems. Recently the animal technicians at our facility collected the blood in 3 mL tubes; we also had blood collected in heparin tubes at the same time. Our stained cells from our EDTA tubes appeared to have signal quenching compared to our heparin tubes. The profiles were unlike any that we had seen before: CD3 and CD20 were spread out and not tight- however samples from heparin tubes were like what we normally see. Has anyone else ever had a similar type problem or know why we would have problems all of a sudden with a smaller EDTA tube? Deborah LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately.
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