Dear Analia,
intracellular staining of cytokines (or other proteins) is no problem in a
lot of cell types. But which lung cells are you talking about. Is it lavage?
Is it lung tissue? Are the cells stimulated in a special way?
With a bit more information I might be able to help you specifically. You
can find more information at
http://home.wanadoo.nl/flowcytometry
Don't hesitate to contact me with specific questions. I know of papers on
the staining of human lavage cells. Do a Pubmed search.
Success,
Joost
____________________________________________________
J.H.N.Schuitemaker
Laboratory Manager / Senior Research Technician
Cellular Immunology Group
Cell Biology & Histology
Academic Medical Center
P.O.box 22700
1100 DE Amsterdam
The Netherlands
Telephone +31 (0)20 5664960
FAX +31 (0)20 6974156
Internet: http://www.amc.nl
http://home.wanadoo.nl/flowcytometry
-----Original Message-----
From: Analía Rial [mailto:arial@higiene.edu.uy]
Sent: maandag 21 oktober 2002 17:12
To: cyto-inbox
Subject: Intracellular cytokines
-- [ From: Analía Rial * EMC.Ver #2.5.02 ] --
Hi, I wanted to ask for some help on this issue. I want to start doing
intracellular cytokine detection by flow cytometry, on murine lung samples
and I wanted to know about a good protocol for doing this, since I know
there exist some tips that could be very helpful for me.
The antibodies we have are from Pharmingen.
Any help would be really appreciated.
Thanks
Analía Rial
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