We routinely set an enumeration gate to ensure that sufficient CD45+ nucleated cells are acquired to ensure 100 CD34+/SSC low cells are included. This guarantees a cv of 10% (using Poisson) which we regad as acceptable. If we can't get 100 CD34+ events then the donor/patient hasn't mobilised anyway Dr Mark W Lowdell Senior Lecturer in Haematology / Director of Laboratory of Cellular Therapeutics RFUCMS T - 020 7830 2183 F - 020 7794 0645
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