Dear Flowers, We have successfully used CFSE with human lymphocytes and mouse spleen cells. We are currently trying to stain K562 cells with CFSE. We tried a broad spectrum of concentrations (0.5 µM to 5µM) and always get intense fluorescence, K562 cells go on dividing, but we do not see more than one peak of green fluorescence even after several days when cells should have passed several cycles. Can anybody explain? Thank you in advance, and kind regards to all Helga __________________________________________________ Dr. Helga Tuschl Österreichisches Forschungszentrum Seibersdorf Ges.m.b.H. Geschäftsfeld Toxikologie A-2444 Seibersdorf Telefon: +43/0 50550 - 3510 Fax: +43/0 50550 - 3653 E-mail:helga.tuschl@arcs.ac.at Web: http://www.arcs.ac.at/L/LT...
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