Hi, Has anyone had flow experiences with Molecular Probes' product MitoTracker Red (CMXRos), in particular with 3-colour staining? We are using a FACSCalibur with a 488 nm laser and find that the fluorescence of CMXRos alone is detected in FL2, FL3 and FL4. We can decrease the FL2 signal to a negative level using FL2 - 99.9% FL3 compensation while retaining the signal in FL3 and FL4. However, we cannot decrease the signal in FL3 using FL3 - 99.9% FL2 (or FL4) compensation. I am having a hard time conceptualizing this compensation model. Any ideas? Thanks so much. Annie Bang Platelet Research Laboratory St. Michael's Hospital Room 2-001 Cardinal Carter Wing 30 Bond Street Toronto, Ontario M5B 1W8 Canada 416-360-4000 ext 2192 416-864-3060 fax banga@smh.toronto.on.ca
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