4/23/2002 Ryan, I can think of two approaches to sorting human chromosome #9. 1. Use a human rodent hybrid containing chromosome #9 (Human Chromosome-Specific DNA Libraries: Construction and Availability, M.A. Van Dilla, L.L. Deaven, ..., Bio/Technology Vol. 4 June 1986 pp537-552), or, 2. Counterstain using DAPI (Counterstaining Human Chromosomes for Flow Karyology, Julianne Meyne,..., Cytometry 5:580-583 (1984)) Joe Fawcett >Quick Question for those who have done some human chromosome sorting... > >Is it possible to separate human chromosome 9 from 10-12? In looking at >papers with the normal Chromomycin A3 versus Hoechst bivariate histogram, >and it always shows a 9-12 cluster. If anyone has discovered another >way(other dyes, reagents, etc...) to separate these, i'd love to know. > > >Thanks, >Ryan
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