Hi All, We are currently setting up a protocol for indirect flow cytometry on the monocytic cell line THP-1. Our primary antibody is raised in goat and we would really like to find a good FITC/PE secondary antibody that has been tried and tested in flow. Any other tips with regard to dilutions etc would also be greatly appreciated. Many thanks in advance, Elaine Kennedy Pharmacology Organon Laboratories Ltd. Newhouse Scotland -------------------------------------------------------------------- This message, including attached files, may contain confidential information and is intended only for the use by the individual and/or the entity to which it is addressed. Any unauthorized use, dissemination of, or copying of the information contained herein is not allowed and may lead to irreparable harm and damage for which you may be held liable. If you receive this message in error or if it is intended for someone else please notify the sender by returning this e-mail immediately and delete the message. --------------------------------------------------------------------
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