Re: Luminescent metals

From: Howard Shapiro (hms@shapirolab.com)
Date: Thu Mar 28 2002 - 17:38:41 EST


Julie Nelson wrote-

>I just finished talking to a researcher who has some exciting ideas
>about detecting luminescent metals using flow cytometry, specifically
>Eu, Tb and Ho.  Has anyone every used flow to analyze these?  Will UV
>excitation (351 nm) work?

These rare earth metals have been used as immunufluorescent labels in
chelated form. Their fluorescent lifetimes are hundreds of microseconds,
making them incompatible with conventional commercial flow cytometers,
although they have been detected in special laboratory-built instruments.

-Howard



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