An interesting question Manfred. I haven't yet come up with a satisfactory method, except to use confocal or laser scanning. Anyone else? Craig ---------- From: manfred@maitz-online.de To: cyto-inbox Subject: Blood platelet activation Date: 14 November 2002 19:59 Dear all, My main interest is to determine the activation of blood platelets when they adhere on a foreign surface (hemocompatibility testing). I measure CD62P on paraformaldehyde and azid treated cells by flow cytometry as a marker for platelet activation (besides cytokine release, morphology, and quantification of adherent platelets). This works fine for the non-adherent thrombocytes, which obviously are also not very activated. Does anyone have an idea how to bring adherent blood platelets to suspension without further activation and with minimal selectivity for less activated platelets? I don't know anything like this from the biomaterials literature. I would be grateful for any suggestions. Best regards, Manfred Maitz -- Manfred Maitz FZ Rossendorf Abt. FWII Postfach 51 01 19 D-01314 Dresden - Germany - Tel.: +49 (0)351/260-2014 Fax.: +49 (0)351/260-2703
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