"Hepatic lymphocytes were isolated by a method described elsewhere (17). The liver was removed and cut in two pieces, one half was passed through 200-gauge steel mesh, and then suspended in RPMI medium with 5% of heat inactivated calf serum. Once the cells washed, the lymphocytes were isolated by a gradient of Percoll (35% Percoll containing 100U/ml heparin, Sigma, St. Louis). Purified lymphocytes were washed three times before culture." 17. Osman, Y., T. Kawamura, T. Naito, K. Takeda, L. Van Kaer, K. Okumura, and T. Abo. 2000. Activation of hepatic NKT cells and subsequent liver injury following administration of alpha-galactosylceramide. Eur J Immunol. 30:1919-28. -- Dr Daniel SCOTT-ALGARA Service d'Immuno-hématologie et d'Immunopathologie Institut Pasteur 75724 Paris Cedex 15 France Phone 33 (0)1 45 68 82 13 Fax 33 (0) 1 45 68 89 51 E-mail: scott@pasteur.fr
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