Sorry about that folks, it seems I forgot to attach the axis labels to the plot. PMT2 is Annexin V -FITC, PMT3 is CD8 - PE. The gate was placed on lymphocytes only not all cells. As it has been asked, I have included all the data plots I have from that sample (1.0 Gy) analysis (suggestions were that it may be bleed over from another channel, that it is over compensated, that the population is too close to the axis thereby throwing off compensation) Original plot 1.0 Gy sample data (See attached file: flow plot.JPG) (See attached file: 1Gy flow.jpg) Thanks again, Barb ____________________ Barbara Kutzner Technologist Radiation Protection Bureau Health Canada 775 Brookfield Road, PL 6303B Ottawa, ON K1A 1C1 (613) 952-9069 FAX (613) 941-1734
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