Hi Allen What sort of pathogens will you be dealing with? I sort a wide variety of pathogens many of which are deadly...to our murine friends. My lab is not negative pressure and I have a custom class I type biosafety cabinet mounted to the sorter. I'd do it differently if I worked with human material or pathogens dangerous to humans. The MoFlo Class I hood thing looks pretty good. Assuming human material and HIV level stuff you would probably want to have the sorter in a dedicated BL-3 room with restricted access with either a Class I hood type arrangement on the instrument, a clean air mask on the operator or both. Just my opinion Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 >>> allen parmelee <allen.parmelee@tufts.edu> - 11/5/02 5:56 PM >>> Hi we're looking into setting up a sorter facility that would handle a wide variety of pathogens. does any one know of any labs set up for this? the only ones i'm aware of are only set up for 1 particular pathogen. also, does any one have any recommendations for design considerations? thanks for any help! have a good one allen -- Tufts Laser Cytometry Allen Parmelee Manager 617-636-0464 allen.parmelee@tufts.edu 617-636-2990 (fax) a core facility offering: MoFlo (Cytomation), FACSCalibur (Becton Dickinson) , LSC (CompuCyte), LCM (Arcturus)
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