Re: Sorting Multiple Pathogens

From: Simon Monard (smonard@trudeauinstitute.org)
Date: Thu Nov 07 2002 - 16:40:37 EST


Hi Allen
What sort of pathogens will you be dealing with?
I sort a wide variety of pathogens many of which are deadly...to our murine friends. My
lab is not negative pressure and I have a custom class I type biosafety cabinet mounted
to the sorter. I'd do it differently if I worked with human material or pathogens
dangerous to humans. The  MoFlo Class I hood thing looks pretty good. Assuming human
material and HIV level stuff you would probably want to have the sorter in a dedicated
BL-3 room with restricted access with either a Class I hood type arrangement on the
instrument, a clean air mask on the operator  or both.
Just my opinion




Simon Monard
FACS Lab Manager
Trudeau Institute
Saranac Lake
NY12983

Ph 518 891 3080 X352


>>> allen parmelee <allen.parmelee@tufts.edu> - 11/5/02 5:56 PM >>>

Hi

we're looking into setting up a sorter facility that would handle a wide
variety of pathogens.
does any one know of any labs set up for this?
the only ones i'm aware of are only set up for 1 particular pathogen.

also, does any one have any recommendations for design considerations?

thanks for any help!

have a good one
allen

--
             Tufts Laser Cytometry

Allen Parmelee          Manager
617-636-0464        allen.parmelee@tufts.edu        617-636-2990 (fax)

            a core facility offering:
MoFlo (Cytomation), FACSCalibur (Becton Dickinson) ,
LSC (CompuCyte), LCM (Arcturus)



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