I have an investigator that is treating cells with BREfeldin A and then examining various intracellular and surface markers. We are getting significant "background" activity or more accurately distinct peaks in a "dim fluorescence" location amounting to around 8-12 %. This activity occurs in all four detectors in the same location and around the same amount. I am wondering if BREfeldin A fluoresces from a 488nm laser? Any thoughts? Thanks in advance. Bill Justice Laboratory Information Systems KU Medical Center Phone: 913.588.3876 Pager: 913.917.7018
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