BREfeldin A - Does it fluoresce?

From: Bill Justice (BJUSTICE@kumc.edu)
Date: Wed Jan 09 2002 - 15:51:16 EST


I have an investigator that is treating cells with BREfeldin A and then examining various intracellular and surface markers.
 
We are getting significant "background" activity or more accurately distinct peaks in a "dim fluorescence" location amounting to around 8-12 %. This activity occurs in all four detectors in the same location and around the same amount.
 
I am wondering if BREfeldin A fluoresces from a 488nm laser?
 
Any thoughts?
 
Thanks in advance.
 
 
 
 
Bill Justice
Laboratory Information Systems
KU Medical Center
Phone:     913.588.3876
Pager:      913.917.7018



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