Brefeldin A should not be fluorescent. However, fixing cells for intracellular staining could significantly increase the background fluorescence. The distinct "dim" peak in all detectors might be dead cells - in any bivariate display, they may form a diagonal tail extending from the main negative population. Bill Justice wrote: > I have an investigator that is treating cells with BREfeldin A and > then examining various intracellular and surface markers. We are > getting significant "background" activity or more accurately distinct > peaks in a "dim fluorescence" location amounting to around 8-12 %. > This activity occurs in all four detectors in the same location and > around the same amount. I am wondering if BREfeldin A fluoresces from > a 488nm laser? Any thoughts? Thanks in advance. Bill Justice > Laboratory Information Systems > KU Medical Center > Phone: 913.588.3876 > Pager: 913.917.7018
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