Strepavidin beads

From: Michael Herron (herro001@umn.edu)
Date: Tue Jun 11 2002 - 12:57:32 EST

Next message: Pizzo,Eugene: "Tumor cell aggregation problem"
Previous message: Josef Azem: "FITC-labelling of bacteria"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

All,

I have started using Dynal strepavidin coated paramagnetic beads to
detect proteins in cell homogenates by flow cytometry.  I am pretty
happy with the initial results, but I was wondering if people could tell
me about the advantages of other beads for such assays.

The paramagnetic nature of these beads make them easy to wash, but they
are quite small (~1 micron?) and I was wondering if larger beads might
have greater binding capacities and give me a lower detection limit for
my protein?

Can anyone inform me?

Thanks,
Mike

--

     ________________________________________________________
    / Michael J. Herron,  U of MN, Dept. of Pediatrics/BMT  /
   / herro001@umn.edu                                      /
  / 612-626-4321   Mpls MN 55455                          /
 /_______________________________________________________/

Next message: Pizzo,Eugene: "Tumor cell aggregation problem"
Previous message: Josef Azem: "FITC-labelling of bacteria"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:12 EST