Hello, I am writing this mail requesting some urgent advice. Please forgive me if this message is long. I work with a Facscan (with a HP computer). Today I was to analyze some samples (estimation of CD4 /CD8/ CCR5/CXCR4 positive cells with the Simulset software). When I ran the calibrite beads (with Autocomp), the PMT voltages could not be set automatically. The event rate was very high at around 1500. I then realized that even when the machine was in "standby" mode, there events were visible on the screen (event rate of around 600). I was able to stop these events by increasing the threshold of Forward scatter from 52 to 100 with the machine on standby. I subsequently ran the samples by manually setting the PMT voltages, the compensation and increasing the threshold. I have two questions. 1. Am I allowed to manually set these values and interpret the results (The Differential count of the samples by the FACScan matches with the differential by a Coulter GenS system) 2. What could be the problem? I have to state that I did open the panels on the sides of the machine and the top cover in order to fix a leak in the fluidics (at the coupling where the tube from the sheath tank is attached - it takes some time for the engineers to come to this part of India). However, I did not touch the flow cell / laser /lenses/ detectors. I need this information as the engineers from BD are unavailable (one person in abroad and the other person is incommunicado) for the next few days. Also some patients have appointments for Tuesday. I would appreciate a response ASAP. I will be out of town from Saturday until Monday and hence tomorrow (Friday) is the only day I have to fix the problem, if minor, before I leave. With regards, Sandeep. ===== Dr. Sandeep Ramalingam, Department of Clinical Virology, Christian Medical College Hospital, Vellore-632004, India. Phone(off): 91-416-222102, ext 2070 Fax: 91-416-232035 Email: sandeeprk@yahoo.com __________________________________________________ Do You Yahoo!? Everything you'll ever need on one web page from News and Sport to Email and Music Charts http://uk.my.yahoo.com
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