Apoptosis in spleen

From: Victor S. Brasil (vbrasil@ms.microlink.com.br)
Date: Tue Sep 17 2002 - 03:22:28 EST

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Dear Flow-ers,

Someone knows a good approach to detect lymphocyte apoptosis in spleen ex
vivo? My experience shows that annexin is a bad choice due to phagocitosis
by spleen macrophages. I think that using PI or tunnel could occur the same
problem (not detectable due to phagocitosis). There is a good reference on
detection of lymphocyte apoptosis in spleen ???
Thanks a lot in advance

Victor Brasil

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