Hi all, We are in the process of getting some extra colours working on our new FACSVantage SE and are trying to find a dye we can excite with the third laser (a mixed gas argon and krypton (Coherent Innova 70 Spectrum UV)). I have read the Molecular Probes literatures and the publication by Telford et al (Cytometry 32:117-125 (2001)) about the use of the ELF-97 enzyme substrate system and was wondering if anyone has any "real-world" experience using it with surface antigens? I am particularly concerned about the 30min incubation at RT that is required. What does this do to live cells? (the MP literature suggests using fixed cells but the Cytometry paper seems to use live cells). What about the labelling of other surface markers? Any other suggestions for alternative dyes that we can use with this laser? Thanks, Adrian Smith -- ______________________________________________ Adrian Smith (Research Officer) T CELL BIOLOGY GROUP Centenary Institute of Cancer Medicine & Cell Biology Locked Bag No.6 Newtown, NSW 2042 AUSTRALIA. Ph: (02) 9565-6198 Fax: (02)-9565-6103
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