Mark Talary asked- >Whilst classically we talk about forward and side scatter as yielding >information about size and granularity, can the side scatter yield >information about the surface roughness or blebbing of a cell membrane or >cell surface ? Yes, it can. However, the side scatter measurement will only help you if only one of these morphologic characteristics differs between cells under different experimental conditions. If there are differences in blebbing and surface roughness and granularity, it will be difficult to figure how much of the difference in signals is due to each factor. -Howard
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