Alexa 532 excitation off an argon 488 laser

From: Calman Prussin (CPRUSSIN@niaid.nih.gov)
Date: Mon Sep 30 2002 - 12:23:35 EST 

I am trying to identify eosinophils by flow cytometry taking advantage of
their avid binding of anionic dyes, such as FITC and Alexa. For one series
of experiments I only have the FL2 channel open on my Calibur to detect the
FITC/Alexa signal. I would like to try and use Alexa 532, which has an
absorption maximum at 532 and emission max at about 550. The FL2 set-up on a
Calibur consists of a 585/42 wide pass.

Spectrum for Alexa 532 can be found here:
http://www.probes.com/servlets/spectra?fileid=11002p72

If bright staining is detected with an optimal flurochrome, what are the
chances I'll get anything usable with the Alexa 532? I guess I just need to
do the experiment, but was wondering if anyone had any thoughts or
experience.

Thanks,

Calman

> _______________________
> Calman Prussin
> Laboratory of Allergic Diseases
> NIAID/ National Institutes of Health

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