Basophils express CD4 weakly - about 1log lower than T cells from the same donor. If you want to determine the frequency of CD4+ cells within the granulocyte cluster you should label the cells with CD4 FITC and then produce a 2-d dot plot of CD4 FITC versus FL2 (assuming this is the orange channel). The autofluorescent eosiniphils from withinth the granulocyte cluster will appear as FL1+/FL2+ whilst the true CD4+ basophils will be FL1+ only (some of the CD4+ basophils will underlie the autofluorescent eosinophils and would be lost without using this method). When I was working in this area some years ago, we found 2-5% of granulocytes from normal blood donors were CD4+ using this technique. Hope this helps Mark Dr Mark W Lowdell Senior Lecturer in Haematology / Head of Laboratory of Cellular Therapeutics RFUCMS T - 020 7830 2183 F - 020 7794 0645
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