Hi, all! In response to my earlier request for the best markers to phenotype human monocytes, several people have asked the flip side of the question--the best markers to identify monocytes/macrophages in the mouse. Because there were so many interested in mouse monocytes, I have decided to reply to the entire group. In our hands, CD11b (Mac-1) and Gr-1 (Ly-6G; clone RB6-8C5) best resolve monocytes/macrophages from granulocytes in the spleen and blood of mice. Both monocytes and granulocytes express CD11b but only granulocytes express Gr-1. So, we routinely include both CD11b and Gr-1 in our stains when we want to enumerate monocytes/macrophages in the spleen and blood. When we phenotype mouse blood, we also include an antibody to CD45 to gate out residual RBCs and platelets. This paper may be of help: Lagasse and Weissman. 1996 Flow cytometric identification of murine neutrophils and monocytes. J. Immunol. Methods 197:139-150 If anyone needs any further information or help with mouse monocytes/macrophages, please feel free to contact me. Thanks! Julie Oughton Oregon State University
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