Thanks Howard I see Coherent has a 20mW 488nm solid state laser, do you know how expensive they are ? How would they perform compared to the 15mW argon lasers in BD benchtops? Simon >>> Howard Shapiro <hms@shapirolab.com> - 4/4/02 8:27 PM >>> Simon Monard wrote- >Has anyone tried putting a flowcell in a FACStar Plus? How hard would it >be to convert it >into a two laser analyser? Does anyone have a quartz flowcell that I could >install? I'm >reluctant to heave it into a dumpster just yet and need an analyser that >can look at >GFP and YFP plus other stuff. Why do you need the quartz flow cell? You will still be stuck with the relatively low N.A. of the FACStar Plus collecting lens, and you won't get problems with scatter measurements from a stream in air if you have the droplet drive turned off. So, unless you really need a closed fluidic system, you might as well use the FACStar as is - well, almost. What I would do is put on a 125 um or larger nozzle; that will give you a bigger stream in air, enabling you to dispense with the blocker bar for fluorescence collection (you may have to kludge up a field stop or two). And the frequency of clogging should go down substantially with the larger nozzle. -Howard
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