Here is a summary of the responses I received to my question "your favorite antibody biotinylation method, reagent, supplier" hi: Sigma's kit & reagents worked well. Remember not to have azide in the buffers during the biotinylation steps. Best, Beverly Beverly E. Barton, Ph.D. Assistant Professor Department of Surgery New Jersey Medical School Box 1709 185 S. Orange Ave. Newark, NJ 07101 phone 973-972-0662 fax 973-972-6803 e-mail bartonbe@umdnj.edu Hi Roger, Any kit you buy from pierce.com will be the best available. I have used their products for 15 years. By far the best. Use thier long arm spacer for best results. Kind regards, Steve McClellan Director of Immunology www.anthrogenesis.com Roger A. Burger, PhD Technical Marketing Scientist Miltenyi Biotec Inc. Auburn, CA (530) 887-5351 NEW<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /> Non-Human Primate Cross-reactivity information for MACS at: http://www.miltenyibiotec.com/index.php
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