What we must know before answer your question is some basic (but
essential) things:
-What type of culture you plan to performed (Semi-solid, liquid
or on stroma).
-What are the phenotype of your "stem cells" population. Some
very immatures one do not growth in vitro.
-What type of sheat fluid you used (preservative or not)
The answer to these questions will induce many various responses
regardind the plating number, the type of medium, cytokine etc..
"Glenda, M, Davison" wrote :
> Dear all flow cytometrists,
>
> We have been trying to sort various sub-populations of CD34+ stem
> cells for culture. The cells were plated at 1x103, however in all
> cases poor growth was observed. Does anybody have experience in this
> area and if so do you have any advice?
>
> Some questions which have been put forward are:
>
> What medium would be ideal to sort the cells into?
> What growth factors would be advisable to add to the culture medium?
>
> Any advice would be welcome . Thanking you in advance.
>
> Glenda Davison
> Dept. Haematology
> Groote Schuur Hospital
> Cape Town
> South Africa
> e-mail: gmd@cormack.uct.ac.za
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