Dear Flowers, I plan to perform flow analysis on murine spleen and lymph nodes (immunophenotyping) and am specifically interested in quantifying the proportion (& absolute numbers) of CD4+ memory T cells, as well as effector (activated) CD4s and naive CD4s. For naive cells, I am condidering using 3G11 in conjunction with CD62L For activated (memory + effector cells) I am considering using CD44 and CD62L. What is the current consensus with regard to identifying memory (CD4+) T cells? Is CD45RB still used? What markers do you suggest I should use for any of these populations (memory, effectors, naive)? Finally, which is the more appropriate way to express this data: as percentages or as absolute numbers (ie., per whole spleen, set of lymph nodes)? Thanks for any help you can provide. Lawrence A. Wolfraim, Ph.D. Laboratory of Cell Regulation & Carcinogenesis (LCRC) National Cancer Institute/National Institutes of Health Building 41, Room B1103 41 Library Drive Bethesda, MD 20892 USA Tel.:(301) 496-2431 Fax (301) 496-8395
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