It is much better to fix the cells after staining in 1-2% paraformaldehyde. This will stabilize the cells for several days. They should be fixed for at least an hour before analyzing. Regards, Tony Bakke >>> maciej simm <flowlab@myflow.net> 04/08/02 07:32AM >>> This is a two parter: 1 - can stained PBMC's be frozen for later analysis? I have people coming in over the weekend who have to stain their experiments. When I acquire these in the morning on Monday, they don't look very good (typically 48-72 hrs after fixing). Is there a way to preserve fluorescence by freezing the cells? 2 - someone in the lab distributed mucho dollars worth of antibodies for 'future stainings' to 'save time'; of course the antibodies dried up into colorful little pellets on the bottoms of the staining tubes. Will these be still usable after mixing them with blood/cell cultures ? or are there irreversible confirmation changes? Thanks for any insights. Maciej -- Maciej S. Simm Senior Technologist Weill Cornell Medical College Pediatrics Hematology/Oncology Immunology Laboratory/Flow Cytometry Unit 525 East 68th Street N-805 New York, NY 10021 voice (212)746 3428 fax (212)746 8573 --
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