Hello I am searching for a 6His tag antibody that works by flow cytometry as a marker for transfection efficiency. I have stably transfected K562 and U937 cells and a mouse fibroblast line and put them under puromycin antibiotic selection and have a population that has grown out but need to prove that they express my protein of interest and aren't antibiotic resistant. What I have seen so far is that the 6-His monoclonal antibodies stain extracellular and permeabilized cells from my parent and transfected cell line equally well. My 6-His tag is expressed intracellular in this case but future studies would place the His tag on the surface of the cells. Are there ways to block "native" HIS expression? Any suggestions would be appreciated. Thank you Shelia Alexander Research Fellow University of Minnesota College of Veterinary Medicine St Paul, MN 55113
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