Dear Flowers, I have a colleague who is transfecting FITC-linked double-stranded RNA (20 base pairs) into cells for flow analysis. His question is whether ethanol fixation would effect the FITC signal either by degrading the RNA or allowing it to leak out of the cell, and I'm not sure. Any help in this matter would be appreciated. Thanks, Yoav -- Yoav Altman Voice: (858) 646-3100 xFLOW (x3569) Manager, Flow Cytometry Core Facility Fax: (858) 646-3167 The BURNHAM INSTITUTE email: <yoav@burnham.org> 10901 North Torrey Pines Road La Jolla, CA 92037
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