We have use 0.1um filter sheath fluid but did not insert it into the fludics. It is very necessary to seperate small bacteria from particulate matter in the sheath fluid. We first filter it through a 0.2um filter followed by a 0.1 um filter. It is SLOW. Tim Chance CDC -----Original Message----- From: Mark Gauci [mailto:markgauci@biotechfrontiers.com] Sent: Thursday, February 28, 2002 7:10 PM To: cyto-inbox Subject: Calibur sheath filter Dear everyone, I was wondering if anyone had any experience installing a finer filter than the standard 0.22um one in the sheath line of a FACSCalibur. If so, what was your experience with this. Thanks in advance, Mark Gauci BTF - Decisive Microbiology www.biotechfrontiers.com
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