Hello, Does anyone know why some mouse Ab isotypes give more background than others when staining human cells? We stained single cell suspensions form human lung enzymatic digests and have a lot of problems with mouse IgG2a antibodies: the isotype control stains very bright. Mouse IgG1 and IgG2b are less of a problem... Could someone explain this selective phenomenon and/or recommend an optimal blocking strategy to get rid of the problem? Thanks a lot, Karim
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