I know this is going to sound like a stupid questions but we(me and several post docs) are currently involved in a heated debate as to the nature and meaning of FL2-area and FL2-width in connection with the measurement of DNA content(PI stained) of ethanol fixed cat lymphocytes. Can someone give us a good definition of each and an explanation of why we use these for DNA measurement instead of FL2-Height? We are using a BD FACSCalibur for our work. I have done DNA work for years and I have never really thought about this before-this is how I was taught to do it. Again I am sure this is result of never having taken a formal course on flow and just picking it up from the person who previously held my job and other investigators. I have searched the archive and can not find a good explanation. Maybe I need a course in using the search engine for the archive!!! Thanks in advance. Janet Dow Janet Dow Research Technician and Manager Flow Cytometry Facility North Carolina State College of Veterinary Medicine Room C-314 Raleigh, NC 27606 (919)513-6364
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