This brings up a very important issue that we platelet types have struggled with frequently. The important flow rate is the total particle flow rate, not the rate of particles "seen" by the machine. Thus the rate that you need to be concerned with is the rate with no thresholding or live gating. Coincidence events often occur between "seen" particles (those above the threshold) and the potential myriad of particles that are not seen, but are still there in the observation volume at the same time that a "seen" particle is present. I don't believe that there is any absolute flow rate that you can use as a guide - you simply have to show that the frequency of the events of interest, or the properties of those events, is independent of particle flow rate - if it is not, then you may be dealing with coincidence. Ken Ault
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:11 EST