Glad to have something to contribute at last........we regularly count
bacteria of 1um size down to 1000 cells/ml, and we use the viability kits
to a similar sensitivity. If you want to just enumerate we have found
SYTO-13 to be excellent and then count on side scatter and FL1, we use a
FACScan. We also use SYTO 13 and a cell impermeable dye like TOPRO (the
dimer, I forget the number) and count on FL1 and FL3 to distinguish
viability. An authority on this is Andy Beavis, who was at Princeton but
has now moved on to a better place. Are you out there Andy.....?
James
Dr. James Hall
Dept. of Geosciences
Princeton University
Princeton, NJ,08544
Phone: (w) 609-258-2597
(h) 609 919 0292
e-mail hall@princeton.edu
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