Dear all for tracking electroporation, you can use the Lucifer Yellow. you stain the cell and after electroporation you have 3 populations: live un-electroporate, live electroporate and dead electroporate. best regards, Marc Barad, Head Cell Imaging Center of Immunology Case 906 13 288 Marseille France www.bdbiosciences.com/.../Products/drug_discovery/pdf/insert_protocol_lucife r_yellow_permeability_assay.pdf http://www.probes.com/handbook/sections/1405.html >>>> Gisele Knowles <gknowles@sten.sunnybrook.utoronto.ca> 09/07/02 >22:39:42 >>> > >Hello colleagues- >Does anyone know of a product that can sit outside a cell and with >either >electroporation or other such stressor, enter the cell and then >fluoresce?? >I have this in my brain that such a thing exists and I'm concurrently >trying >to avoid using fitc dextran altogether because I'm finding too much >non-specific fluorescence. Anyway all comments would be appreciated. >Thanks > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:15 EST