Not exactly what you were looking for, but you can give BrdU in vivo in humans and perhaps follow cell division indirectly. Identification of dynamically distinct subpopulations of T lymphocytes that are differentially affected by HIV. J Exp Med 2001 Dec 17;194(12):1731-41Kovacs JA, Lempicki RA, Sidorov IA, Adelsberger JW, Herpin B, Metcalf JA, Sereti I, Polis MA, Davey RT, Tavel J, Falloon J, Stevens R, Lambert L, Dewar R, Schwartzentruber DJ, Anver MR, Baseler MW, Masur H, Dimitrov DS, Lane HC. > ---------- > From: Norbert Meidenbauer > Sent: Thursday, August 22, 2002 3:33 > To: Cytometry Mailing List > Subject: Proliferation analysis of T cells in vivo > > > Dear flowers, > > we are doing adoptive T cell therapy with antigen-specific T cells > for malignant melanoma, tracking down the transferred T cells with > tetramer-assays. In average, we are seeing frequencies of antigen- > specific T cells between 0,5-2% of CD8+ T cells in the peripheral > blood after transfer. We now would like to know, if the transferred T > cells still proliferate in the patients. > Does anybody now about a dye to label the T cells before transfer, > which is approved for use in humans. CFSE, PKH and the Mitotrackers > are not approved for human use according to the MSDS (as far as I > know). > For any hints and suggestions I would be very thankful. > > > Norbert Meidenbauer > Dept. Hematology/Oncology > University of Regensburg > 93042 Germany > e-mail: Norbert.meidenbauer@klinik.uni-regensburg.de > >
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