Hi! I'm trying to perform a cell cycle analysis of an asssay I made and I don't know which algorithm to use. I have two choices with my Partec PAS: 1) "Fit One Cycle". An algorithm that performs a complete numerical fit (see attached file for results) and 2) "Fit G1/G2M". Only performs a Gaussian fit to G1 and G2/M and analyzes S phase data "as-is" i.e. without fit (see attached file). The two methods both generate results with the same "trend" BUT method 1 shows a constant higher percentage of cells in S phase than cells in G2M whereas method 2 shows the exact opposite. Which one to trust? Method 2 always generates smaller ChiSqu than method 1, even when the peak CVs are quite high (8-10). How high are the CVs "allowed to get"? What can you do to improve them? What's a good ChiSqu? How important it is that the ChiSqu is low? Many thousand thanks in advance to whomever takes of there precious time to help a flow rookie! Best regards, Ulrika K Eriksson Division of Biotechnology Royal Institute of Technology Stockholm Sweden --
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