Fluorochromes choice

From: Michal Abel (abelm@necker.fr)
Date: Wed Oct 30 2002 - 14:21:40 EST


Dear experts in flow cytometry,

I have a possibility to use 6 colors FACS. The trouble is that I am
not sure it is possible with the filters I have.
I would very much appreciate any help.
The instrument has three lasers: 325, 488 and 633 nm and I can use
following filters:
380/LP
400/40
424/44
500/11
510/20
530/28
575/15
610/20
660/13
670/LP
682/13
Is it possible to find 6 different flourochromes, which would be
efficiently differentiated with these filters/lasers ?

Thank you very much for your help.

Michal

--
Michal Abel, MD, PhD
Laboratory of Clinical Immunology
INSERM U25
Necker Hospital, 161 Rue de Sèvres. Paris
tel: 33 1 42 19 28 87
fax: 33 1 44 49 53 74



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