Beth,
> Our lab is going to start using Margaret Goodells method to look at SP
> cells. For those of you already doing this can you tell me what type of
> laser you use for UV? I am looking at using either an Enterprise laser
> with 488nm and UV or having separate UV and 488nm lasers.
>
>
We have used an Enterprise 488/UV for sorting SP cells on our MoFlo and
have done so successfully. However, we find we need pretty high UV
power to get good resolution (particularly in the red). We would like
to use 50mW UV but this is right at the top of the Enterprise's spec.
and after a year or 2 of operation, the spec. may be hard to achieve.
If I were choosing lasers now and *if* mains power and cooling water
were not a problem and *if* I had money for 2 lasers instead of 1, I'd
now prefer an I90 or I300 for the UV and a separate 488nm source.
Frank.
| | < Frank Battye PhD battye at wehi d0t edu d0t au
\__/ <<<<< The Walter & Eliza Hall Institute
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