Dear All, I'm new to this list, but would appreciate any hints people might be able to give me on interpretation of results I am getting. I am measuring Bcl-2 expression in flow cytometry, looking mainly at human peripheral leucocytes and occasional lymphoma samples. I have also tried the Daudi cell line, which I am told should be negative(?). I have been permeabilisng cells with Fix and Perm from Caltag My real problem is that I get really inconsistent results with different antibodies from different places. I have tried FITC conjugated antibodies from Caltag and Dako, and get very different results - basically everything is positve using the Caltag reagents and weak or negative using Dako reagents. I've tried titrating the reagents - and both titrate very quickly giving half log reductions in intentisty with only 1/2 dilutions. I know they are different antibody clones - but I don't know what the "true" results are. I would be really grateful for any advice - especially if anyone knows a really good control system. Thanks in advance for any suggestions, Andy Baker Imperial College London _________________________________________________________________ Get your FREE download of MSN Explorer at http://explorer.msn.com/intl.asp.
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