I am a new user of a new MoFlo. We are working on separating SP cells in bone marrow, which seems to be working well. The problem I am having are simple antibody staining. We use several stem cell markers conjugated to FITC and PE. What we usually get is a long tail of positive stained cells that go off scale, or an un-separatable population. The attachment shows both types, Sca1 and CD34 respectively. We have another brand new MoFlo on campus which gave similar results for ckit. We are having a devil of a time separating these populations. I don't know if it's a settings, hardware or antibody problem. But I'm at the end of my minimal rope. Any suggestions? Tim Tim Macatee Department of Internal Medicine Division of Cardiology UT Southwestern 6000 Harry Hines Blvd. Dallas, TX 75390-8573 (214) 648-1175
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