Hi Jonni, Platelets interact with monocytes through a number of receptor-ligand pairs. MAC-1 does interact with platelet GP1b and fibrinogen. Some antibodies to MAC-1 block these interactions and inhibit monocyte-platelet aggregate formation. Therefore, using an antibody to MAC-1 is not an optimal choice. We have used CD14, CD45 or CD64 with light scatter parameters to identify monocytes with good results. Platelet specific identifiers CD41, CD61, CD42a, or CD42b are used to identify adherent platelets. See "Current Protocols in Cytometry" chapter 6.10 (2002) for guidelines in preparing samples for platelet analysis by flow. Lori Krueger Center for Platelet Function Studies University of Massachusetts Medical School ----- Original Message ----- From: "Jonni S Moore" <moorej@mail.MED.UPENN.EDU> To: cyto-inbox Sent: Saturday, August 03, 2002 11:32 AM Subject: Platelet-monocyte aggregates > > > Hi all, > > We have a client who wants to identify platelet-monocyte aggregates..he thinks > he wants to use double labeling with MAC-1 and probably GPIIb/IIIa...anyone done > this and is this the best way??? > > thanks > > Jonni > > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:18 EST